Matthew C. Difazio received his B.A. degree in biology from Johns Hopkins University in Baltimore in 1988 and received his M.D. degree from the University of Michigan in Ann Arbor in 1992. He completed his residency in diagnostic radiology from 1992-1996 at the University of Chicago Hospitals where he was elected co-chief resident in 1995. He completed his fellowship in MRI, CT, and ultrasound at Thomas Jerfferson University Hospital in Philadelphia, Pennsylvania in 1997.

Dr. Difazio received specialty certification in diagnostic radiology from the American Board of Radiology in 1996. In 1997, he joined the Jameson Health System, Inc. in New Castle, Pennsylvania as an attending radiologist. Dr. Difazio joined Princeton Radiology Associates in 1998.